Long non-coding RNAs (lncRNAs), categorized as non-coding RNA, are a class of transcripts with low sequence conservation in different species, a length of over 200 nucleotides, and no protein-coding ability. It has long been widely believed that non-coding RNAs (ncRNAs) do not encode proteins. Research on plant lncRNAs is just beginning and has gradually emerged in plant molecular biology.

Lifeasible provides analysis services for plant lncRNA non-coding ability validation, including prediction methods, vector construction, immunofluorescent staining, western blotting, and others, to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Prediction Methods

• Predicting the coding ability of lncRNAs is a time-saving method and provides direction for our experimental validation.
• We provide analysis services for plant lncRNA non-coding ability validation by prediction methods. It is first necessary to determine whether the LncRNA has an open reading frame (ORF) and then to predict the coding ability.

Vector Construction

• The above is only the predicted result; in order to further test experimentally whether it has the coding ability, the relevant vector is constructed and see whether it expresses the corresponding protein in vivo.
• We help our customers construct plasmids containing peptides and lncRNAs to verify their ability to encode. To obtain more reliable experimental results, we also design rigorous experiments and construct vectors with ORF C-terminal fusion tags separately while mutating the ORF start codon ATG to ATT as a negative control.

Immunofluorescent Staining

• Immunofluorescent staining (IF) is a technique that uses antibodies and fluorophores to mark specific proteins. Scientists can use IF to identify different cell types, track the location of proteins throughout the cell, and determine protein co-localization.
• We provide analysis services for plant lncRNA non-coding ability validation by IF staining, which consists of the following six basic steps, fixation, permeabilization, blocking, primary and secondary antibody incubation, and image acquisition.

Western Blotting

Fig. 1 Western blotting procedure.

• Western blotting is a laboratory technique that uses specific antibodies to identify proteins that have been separated based on size by gel electrophoresis. The method involves using gel electrophoresis to separate the sample's proteins. The separated proteins are transferred from the gel to the surface of a membrane.
• We help our clients analyze plant lncRNA non-coding ability validation by western blotting, which includes the following workflows, sample preparation, gel electrophoresis, protein transfer, blocking, primary antibody incubation, secondary antibody incubation, and protein detection & analysis.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please contact us or make an online inquiry.

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Related Services

• Plant lncRNA Expression Validation Analysis
• Plant lncRNA Localization Validation Analysis
• Plant lncRNA Interaction Validation Analysis