Sequential Window Acquisition of all Theoretical fragment ions (SWATH) is a new mass spectrometry acquisition mode technology. As a DIA (Data Independent Acquisition) technology, SWATH divides the whole scanning quality range of MS into several small windows and splits all the ions in each window in turn, so that all the peptide ions in the scanning range can be scanned by high-speed first-order MS and then analyzed by secondary MS/MS. Therefore, SWATH can quantitatively determine almost all detectable molecules in complex samples. SWATH combines the characteristics of high-throughput detection of shotgun proteomics and the advantages of accurate quantitative analysis and is a real panoramic mass spectrometry detection method MS/MS ALL.

What We Offer

Lifeasible adopts a high-resolution mass spectrometry system, which has the ability of fast scanning speed and quantitative sensitivity of series quadrupole mass spectrometry system, combining the high resolution, accurate mass number stability, high sensitivity, and high-speed scanning characteristics of mass spectrometry system, launch of SWATH quantitative proteome analysis service technology. You only need to send your sample to us, and we will be responsible for all the follow-up matters of the project, including protein extraction, protease digestion, peptide separation, mass spectrometry analysis, mass spectrometry raw data analysis, bioinformatics analysis.

Service Flow

• SWATH-MS workflow and protocol

• Improvement for SWATH-MS analysis

Technical Characteristics

• Quantitative identification of all molecules in complex samples, including low abundance proteins and peptides.
• The quantitative accuracy is high (equivalent to that of MRM), but there is no need to debug the sample determination method in advance as MRM does.
• More than 2000 proteins can be quantitatively detected in one experiment.
• Complete and comprehensive information storage, which can store the data of each peak of MS, MS/ MS, and there is no need for repeated detection of the same sample.

Reference

1. Mo X. C., et al. "SWATH-MS-Based Proteomics: Strategies and Applications in Plants." Trends in Biotechnology, 2020, https://doi.org/10.1016/j.tibtech.2020.09.002.

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For research or industrial use.

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