High plasticity of cell differentiation is one of the central features of plant cells. Plants respond to stresses such as trauma or pathogen infection by producing disorganized clusters of cells, such as calluses or tumors. Callus can arise from individual differentiated cells; many callus cells are totipotent and capable of regenerating entire plant bodies. Under certain conditions, callus cells also undergo somatic embryogenesis, the process of producing embryos from adult somatic cells. Thus, some forms of callus formation are thought to involve cell de-differentiation. Callus culture facilitates the expansion of restricted plant material. In addition, plants regenerated from callus can isolate rare somatic asexual lineage variants that arise either from existing genetic variation in somatic cells or from mutations, chromosomal aberrations, and epigenetic changes induced by in vitro-imposed environmental stimuli (including growth factors added to cultured cells).

Fig. 1. Schematic Illustration of various types of plant callus. (Ikeuchi et al., 2013)

What We Offer

Based on years of experience in plant tissue culture, Lifeasible provides professional callus culture solutions for customers worldwide. Our callus culture solutions include the induction of callus in growth hormone-containing media. Stem regeneration is induced by decreasing the amount of growth hormone in the medium and increasing the amount of cytokinin in the medium. Elongated shoots are usually transferred to the root-inducing medium, but flowering and seed setting can be carried out in test tubes even in the absence of roots.

• Selection of Material for Callus Culture

Almost any part of the plant can be used to culture healing tissue, such as stems, leaves, hypocotyls, and root explants. We will select the appropriate material according to the requirements of the project. If the explants extracted from the plant tissue are obtained under sterile conditions, a microbial infection is avoided, and the solid gel medium of growth hormone (such as growth hormone cytokinin) is added.

• Induction of Callus
• Wound-induced callus: The formation of callus in different parts of the plant is promoted by trauma. Wound-induced calluses originate from various cell types, including vascular cells, cortical cells, and myeloid cells. This method can be used in various situations, such as tree debarkation and horticultural propagation. These calluses often accumulate plant antitoxins and pathogen-associated proteins that protect against infection and water loss.
• Phytohormone-induced callus: We offer a combination of two growth-promoting hormones, growth hormone, and cytokinin, to induce callus in plant explants in vitro. This method can be widely used in basic research and horticultural applications.
• Callus formation by regaining embryonic or meristematic tissue fate: Ectopic overexpression of embryonic regulators or meristematic tissue regulators has been used to induce callus formation in various plant species.

• Callus Culture

We offer well-established culture media for healing tissue culture, including Murashige and Skoog (MS) medium, White medium, and xylem medium. Laboratory conditions for maintaining healing tissue cultures vary from species to species, and we equip ourselves with the optimal conditions for each situation. We usually add specific phytohormones to the medium to stimulate healing tissue growth.

Advantages of Callus Culture

• Callus culture can be maintained indefinitely in vitro by periodic passaging of cells.
• Callus cells resemble undifferentiated meristematic cells; among other characteristics, they display only small vesicles and lack chloroplasts for photosynthesis.
• Maintained under an appropriate growth medium different from the standard medium, callus culture can redifferentiation into whole plants.
• Callus culture can usually be grown under specific diurnal conditions (e.g., 16 hours of light, 8 hours of dark, 25 ± 2 °C).

Applications of Callus Culture

• Production of secondary metabolites for therapeutic purposes
• Production of therapeutic antibodies
• Production of agricultural plants
• Production of horticultural plants

We Do Better

Lifeasible is committed to developing the full potential of callus plant culture technology. We hope to use gene technology techniques to transgenic callus cultures to synthesize bioactive secondary metabolites and produce plants with improved salt, drought, disease, and pest resistance. We are based in the plant technology industry with professional and fast technical services, and our strict system and mature platform guarantee the success of your project.

Reference

1. Ikeuchi, Momoko, Keiko Sugimoto, and Akira Iwase. "Plant callus: mechanisms of induction and repression." The plant cell 25.9 (2013): 3159-3173.

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For research use only, not intended for any clinical use.

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