Polyploidization is a natural phenomenon in plant evolution and a significant driver of plant evolution. Epigenetic phenomena exist in natural and synthetic heteropolyploid plants, which can give rise to diversity of expression, genetic and cytological diploidisation, and genomic coordination in polyploid plants after gene doubling. Polyploidy is an important area of plant epigenetic research. Lifeasible uses techniques such as population genetics and multi-omics analysis to identify epigenetic loci associated with important agronomic traits in polyploid crops. We are also available to meet your needs for further crop improvement through artificial design and epigenetic breeding and to explore the creation of new polyploid species.

Effects of polyploidy on gene expression

We combine general transcriptome sequencing, lncRNA sequencing, small RNA sequencing, and other methods to reveal transcriptional regulatory mechanisms, enabling you to analyze data such as gene silencing frequency and gene expression preference.

Effects of polyploidy on genomic methylation

Using high-throughput sequencing-based assays, we can analyze the differences between diploid plants and their homologous tetraploids to obtain differences in the proportional distribution of different methylation types between the two ploidies. For comparison, we can also dilute, segregate, amplify, and construct a set of transformed libraries from bisulfite-transformed genomic DNA and sequence them to obtain genomic haplotype methylation information.

• We use high-throughput sequencing with shorter read lengths to interpret haplotype methylation information of long fragments of genomes.
• It is suitable for haplotype methylation analysis of genomes with low heterozygosity and other genomes with high heterozygosity or polyploidy.

Effects of polyploidization on chromosomes

Our use of HI-C, ATAC-seq, Chip-seq, and BS-seq techniques allowed us to identify chromosomal differences that occur in homotetraploid plants compared to their diploid ancestors.

Comprehensive analysis of cases

We can provide a comprehensive overview of the epigenome mapping of heteropolyploid plants, the epigenetic basis of the imbalance between subgenomes, the epigenetic differences between different types of duplicated genes, and the critical role of newly identified chromatin states in the tissue-specific expression of genes in heteropolyploid plants.

• We can systematically analyze epigenetic modifications between different subgenomes of heterozygous polyploid plants. To identify expression bias of homologous gene pairs between different subgenomes and epigenetic modifications of homologous genes with high expression levels.
• We can define different chromatin states in epigenomic mapping and identify new chromatin states by validation of ChIP-reChIP experiments.

Epigenetic modifications such as DNA methylation, IncRNAs, miRNAs, etc., do not respond to plant polyploidization independently but regulate each other synergistically. Lifeasible can use molecular genetics to analyze more heterologous polyploid plant taxa at different stages of evolutionary history to help further you reveal the mechanism of epigenetic phenomena such as gene silencing and genomic imprinting during polyploid formation and their role in polyploid genome evolution. Please feel free to contact us to submit your request.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

Related Services

• Epigenetic Analysis at the Histone Level
• Epigenetic Analysis at the Chromatin Level
• Epigenetic Analysis at the RNA Level
• Analysis of Epigenetic Molecular Mechanisms
• Epigenetic Editing Breeding Techniques
• Epigenetic Analysis at the Methylation Level