Histone is the major structural protein in eukaryotic chromatin, and its N-terminus can undergo various chemical modifications, such as acetylation, methylation, phosphorylation, etc. These modifications change the chromatin conformation, which in turn affects the transcriptional activity of genes. Histone lactylation is a common post-translational modification, which refers to altering the charge and structural properties of histones by covalently binding lactic acid molecules to lysine residues of histones. This type of modification plays a key role in the cell nucleus, and as an important type of modification, it is involved in the regulation of plant growth, development, response to adversity and other key processes. Accurate detection of histone lactonization levels is essential for an in-depth understanding of these physiological processes.

Relying on advanced technology, Lifeasible provides a high-precision platform for the detection and analysis of histone lactylation in plants, which can identify the precise location of lactate modifications, evaluate their dynamic changes under different conditions, and reveal their effects on protein structure and function. We can provide comprehensive, efficient and customized analysis solutions to meet your needs in plant histone lactylation detection.

Plant histone lactylation Assay

• Antibody-specific recognition. lactylation-specific antibodies are a commonly used detection tool for lactylation-modified histones by immunoblotting, immunohistochemistry and other methods. These antibodies are highly specific and can recognize lactylation sites, thus helping researchers understand the distribution and abundance of lactylation.
• Mass spectrometry. Mass spectrometry is a highly sensitive method for the detection of lactylation histones, which allows for the identification and quantification of lactylated histones through the high resolution and accuracy of mass spectrometry instruments. Through comparison with databases and quantitative measurements, a full picture of lactylation histones can be obtained and further analyzed for their association with diseases.
• Immunoprecipitation. Immunoprecipitation is a technique in which histones of lactylation are conjugated with specific antibodies and then isolated by precipitation. This method helps researchers to isolate and characterize histones of lactylation and further study their biological functions in diseases.

Advantages of plant histone lactylation detection technology

Mass spectrometry can achieve high-throughput and high-sensitivity analysis of histone lactate modification sites, and can also detect multiple modification sites at the same time, quantitatively analyze the changes in histone lactate levels, and combine with bioinformatics analysis to predict the regulatory mechanism, which provides powerful technical support for elucidating the role of histone lactate in plant physiology.

• High selectivity. We can accurately and reliably detect lactate modification status in plant cells by using specific antibodies or other molecular tools that recognize lactate modification.
• High sensitivity. Mass spectrometry can accurately and sensitively detect lactated modifications in proteins at very low abundances.
• High-throughput. We offer mass spectrometry that provides rapid and high-throughput detection of lactated modification states in samples and simultaneous detection of multiple histone modifications, providing a comprehensive modification profile.
• Quantitative capabilities. Our quantitative strategy of labeled and unlabeled mass spectrometry allows us to assess the relative or absolute abundance of lactylation modifications to understand the impact of changes in modification dynamics on biological processes.

Our technical process of plant histone lactylation assay

• Tissue sample extraction. Total proteins are extracted from plant tissues with appropriate processing, lysis and purification steps to obtain histone samples.
• Enzymatic digestion. Enzymatic digestion of histones using specific enzymes to obtain peptides.
• Liquid chromatography separation. The enriched peptides were further separated using high-performance liquid chromatography.
• Peptide enrichment. Peptides containing lactated modifications are enriched using specific antibodies.
• Mass spectrometry detection. The enriched peptides were analyzed by LC-MS/MS to identify and quantify the lactate modification sites.
• Bioinformatics analysis. The effects of lactation modification on histone structure and function were predicted by combining with protein databases.

Plant histone lactylation assay in plant research

• Plant adversity response research. Plant cells undergo many protein modification changes after being stimulated by adversity (e.g., high salt, drought, low temperature, etc.). Detection of lactylation can reveal the potential regulatory mechanisms in the plant adversity response network, which can help to understand the process of plant adaptation and response to adversity.
• Crop improvement. Through the study of lactonization, researchers can uncover key gene regulatory mechanisms that affect crop traits (e.g., disease resistance, stress tolerance, etc.) and provide new epigenetic markers for crop breeding.
• Explore plant growth and development. Studying the dynamics of lactonization in different developmental stages of plants (e.g., seed germination, floral organ formation, etc.) can explore their roles in developmental regulation.

By analyzing the dynamic changes of histone lactylation at different developmental stages and under stress conditions, we can reveal the molecular mechanisms involved in the regulation of gene expression. Genetic engineering can also be used to alter the key lactonization sites and study their effects on plant phenotypes, providing new strategies for crop molecular breeding.

Our service workflow

Advantages of our services

• High Accuracy. We use a high-precision resolution mass spectrometer, which can detect peptide sequences with high accuracy, with 90 minutes of on-board time and higher peptide coverage.
• High efficiency. Our advanced technology platform ensures a short testing period, we can provide the test report in about a week.
• Professional technical team. Our research team has rich service experience in plant histone lactate modification assay to ensure the success rate of the experiment.

Lifeasible is committed to the forefront of plant research, and our advanced experimental platform allows us to perform high-quality plant histone lactonization assay analysis for our clients. The technical services we provide offer new perspectives in helping researchers gain a deeper understanding of plant life activities. If you are interested in us, please feel free to contact us.

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