Plant genetic transformation technology introduces target genes into the host plant and expresses them in the plant to produce plants that meet human needs in terms of agronomic traits, resistance, and nutritional quality. Compared with conventional breeding techniques, plant transgenic technology breaks the isolation between species, has higher predictability, can significantly accelerate the breeding process, and can adapt to the current changes in the natural environment. Plant transgenic technology is one of the most revolutionary contributions to crop variety improvement and production in the last three decades. Transgenic soybeans, maize and oilseed rape with insect and herbicide resistance as the main traits have produced tremendous benefits for high, stable, and efficient agricultural production.

What We Offer

Currently, Lifeasible has established a series of plant genetic transformation methods such as Agrobacterium-mediated, gene gun, pollen tube passage, polyethylene glycol-mediated, and electrokinetic methods. In addition to conventional antibiotic and herbicide resistance genes, visualization of marker genes such as anthocyanins and red fluorescent proteins is also easier, faster, and safer for plant selection. In terms of species, we have successfully established transgenic systems for cotton, soybean, corn, rape, rice, alfalfa, tobacco, papaya, potato, tomato, flax, sunflower, banana, sugar beet, bell pepper, strawberry, and melon.

Transgenic methods	Characteristics
Agrobacterium-mediated method	Simple operation, short cycle time, high transformation rate, mature and reliable method, little gene silencing, short transgenic cycle, large transformed fragments, and obviously inserted fragments. Only dicotyledonous plants are infested under natural conditions, which limits their application in cereals and grains, and too many factors need to be considered in the experimental design stage.
Particle bombardment method	Simple operation, short transformation time, large quantity, almost no requirement for recipient plants, large transformable gene fragments, not conducive to stable expression and inheritance of exogenous DNA, the high mutation rate of progeny, low transformation rate, expensive equipment.
Pollen tube passage method	Large quantity, no species requirement for recipient plants, no tissue culture process, unclear mechanism, lack of molecular biological evidence, restricted by natural conditions, poor reproducibility.
Electroshock method	No host restriction, simple operation, too long cycle time, low transformation efficiency, expensive equipment.
PEG-mediated transformation method	Simple operation, widely used and the relatively high prospect of application requires protoplasts and high environmental requirements.

Service Flow

We Do Better

Lifeasible has ranked among the industry leaders in gene cloning, transgenic product development, and industrialization capabilities, and has formed a stable transgenic product development team. Based on the totipotency of plant cells to regenerate plants, we use mediated or other physicochemical methods and techniques to introduce exogenous genes into recipient cells and integrate them into the genome to obtain complete plants through tissue culture. To screen positive transgenic plants in culture, plant-sensitive antibiotics are often used, and finally, molecular biology and physiological tests are performed

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For research use only, not intended for any clinical use.

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