Methylation modification is one of the most common post-translational modifications of plant proteins and is widely found in the eukaryotic proteome. Methylation occurs mostly on transcription factors and histones, and a small number of cytoplasmic proteins are also subject to methylation modifications. Methylation can occur on arginine and lysine residues, including monomethylation, symmetric/asymmetric dimethylation, and trimethylation. Arginine methylation regulates RNA processing, gene transcription, DNA damage repair, protein translocation, and signal transduction. Lysine methylation regulates histone function and participates in the epistatic regulation of gene transcription. Studying the plant methylation proteome can help reveal the role of plant protein methylation.

What We Offer

Mass spectrometry can be used for plant proteomics methylation modification analysis studies to characterize and quantify plant methylated proteome and find differential proteins, thus helping to reveal the role of plant protein methylation. Lifeasible provides mass spectrometry-based services for the identification and quantification of plant proteomics methylation modifications. After enrichment of methylated peptides using specific antibodies against different methylation sites and modification forms, combined with LC-MS/MS analysis, quantitative characterization of large-scale methylated proteins can be achieved.

Service Flow

We Do Better

• We use 2-3 enzymes for protein digestion to ensure 100% coverage.
• High identification throughput, up to over 25,000 methylation modification sites in a single run, identifying and quantifying up to thousands of proteins.
• We have advanced and highest resolution and sensitivity mass spectrometer, complete mass spectrometry platform including MALDI-MS, HPLC, etc.
• We use high-quality antibodies with high specificity for the analysis of low abundance methylation modified proteins.

Reference

1. Roberto B., et al. "Histone Methylation - A Cornerstone for Plant Responses to Environmental Stresses?" Abiotic and Biotic Stress in Plants - Recent Advances and Future Perspectives. 2016, DOI: 10.5772/61733.

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