Haploids contain only one set of genes and are therefore good materials for studying the nature and role of genes, for screening mutants at the cellular level, and for screening plant genetic clones. In haploids, the role of recessive genes is not disturbed by dominant genes, and once a favorable mutation occurs in the donor plant, it can be expressed in the haploid, and the variation can be retained to form new germplasm resources.

At the same time, haploid material can identify the chromosome group composition of the original plant parents, which can be used to study the kinship of different species and the evolutionary process of species. In addition, DH populations can be used to construct linkage maps of plant genes. In addition, crosses between haploids and diploids of the same species can yield a rich variety of aneuploids, which can be used to determine the role of linkage groups, diploid chromosome composition, and gene dosage, and are of great theoretical and applied significance for plant fast-breeding, germplasm conservation and breeding for superior traits.

What We Offer

The extremely low frequency of spontaneous generation of haploids in nature greatly limits the application of haploids. Lifeasible uses artificially induced haploid plants for doubling to form double haploid plants and establish DH lines, which can rapidly purify the genotypes of the parents. We can perform haploid plant induction by several methods.

• Using the isolated male nucleus development pathway, i.e., isolated anther or pollen (i.e., microspores) culture.
• Using the in vitro gynogenesis pathway, i.e., unpollinated ovaries or ovules in vitro culture and radiation pollination-induced solitary reproduction.
• Using the in vivo gynogenesis pathway, i.e., induction of solitary reproduction by pollination with distant pollen.

Lifeasible has successively obtained unpollinated ovary or ovule cultured haploid plants in 10 families and 25 species and 2 varieties in tobacco, wheat, barley, oat, rice, maize, sunflower, gerbera, little sunflower, lily, onion, potato, sugar beet, zucchini, strawberry, white konjac, mulberry, poplar, etc. Our team has extensive research experience and proven processes to meet your various project needs.

Reference

1. Li F., et al., “Haploid induction via unpollinated ovule culture in Gerbera hybrida.” Scientific Reports, 2020, 10: 1702.

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