Disulfide bonds are protein post-translational modifications formed by cysteine residues inside proteins and are widely found in proteins such as hormones, enzymes, immunoglobulins, and plasma in prokaryotes and eukaryotes. The disulfide bond, as a covalent bond that glues two cysteines within or between polypeptide chains, plays a pivotal role in stabilizing the spatial structure of proteins, maintaining the correct spatial folding conformation, and maintaining and regulating the biological activity of proteins. Therefore, identifying the disulfide bond positions of plant proteins is important for identifying the primary structure of plant proteins and helps to reveal the advanced structure and biological functions of proteins.

What We Offer

Lifeasible introduces plant protein disulfide bonding analysis solutions using an advanced and accurate mass spectrometry platform combined with Nano-LC. Lifeasible' extensive experience in protein disulfide bond analysis at the individual protein and histological levels includes sample preparation methods to overcome protein disulfide bond exchange in vitro and maintain its natural structure, efficient mass spectrometry methods for disulfide bond cross-linked peptides, and accompanying disulfide bond identification software. All you need to do is tell us your experimental objectives and send us your samples, and we will take care of all the follow-up of the project and provide information about the protein disulfide bond sites.

Sample Requirement

• Plant leaves, rhizome, xylem, bast >3 g
• Total protein >200 ug

* If you are providing tissue samples, please send us the tissue samples on dry ice.
* If you are providing protein samples, protein extraction can be done with normal tissue and cell lysis solution.

We Do Better

• Different types of analysis: different types of disulfide bond formation can be analyzed.
• High precision and accuracy: accurate identification of disulfide bond peptide sites, excluding the influence of false positives, high accuracy of identification results.
• Complete solution: provide a complete solution including sample preparation.

Reference

1. Lee K., et al. "Defining the plant disulfide proteome." ELECTROPHORESIS. 2004, 25: 532-541.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

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