Glycerol esters are important primary metabolites in plants and are involved in the formation of cell membrane lipids. Aliphatic triglycerides are classified as triglycerides (TG), diglycerides (DG), and monoglycerides (MG) depending on the number of fatty acids bound to the glycerol carbon skeleton. There are various methods for the analysis of vegetable glycerides, among which chemical methods and column chromatography are error-prone, time-consuming, and labor-intensive, while enzymatic methods are prone to acyl transfer during hydrolysis, leading to misidentification of fatty acid positions. The instrumental method has the advantages of high sensitivity, short time, small sample size and high efficiency, and is the most commonly used method for lipid analysis.

What We Offer

Lifeasible provides the analysis of plant glycerides by chemical methods (monoglycerides by periodate), column chromatography, enzymatic methods, thin layer chromatography (TLC), gas chromatography (GC), high-performance liquid chromatography (HPLC), mass spectrometry (MS), and nuclear magnetic resonance (NMR).

• High-performance liquid chromatography (HPLC)

The detectors used for HPLC are generally ultraviolet (UV), differential (RI), and evaporative light scattering detector (ELSD), while mass spectrometry (MS) detectors are commonly used for qualitative components. RI and ELSD are general-purpose detectors, and the relative quantification of each substance can be achieved based on the ratio of their peak areas, but the RI detector can only be analyzed with an equal gradient mobile phase, while ELSD can be suitable for gradient elution and has high detection sensitivity.

• Thin-layer chromatography (TLC)

Compared to HPLC, its greatest advantages of ELSD over HPLC are that it is intuitive, fast, widely used, low cost, and especially suitable for the simultaneous detection of a large number of samples.

We Do Better

Lifeasible provides a full platform of plant glycerides analysis and will select the appropriate analytical method according to the composition and precision requirements of the sample provided by the customer, which can effectively separate phospholipids and glycolipids in trace tissue samples, providing a simple and effective service for researchers studying plant lipid metabolism. In the HPLC-ELSD analysis, we can separate glycerides of different fatty acid compositions by selecting the appropriate eluent and using a suitable gradient elution procedure, and at the same time, we can achieve a good separation degree and ensure that the peaks can be produced within a reasonable time frame.

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For research or industrial use.

Related Services

• Plant Free Fatty Acid Analysis
• Plant Whole Lipidome Analysis
• Plant Phospholipid Analysis