Long non-coding RNAs (lncRNAs) are RNAs of more than 200 nucleotides in length that are not (or very rarely) translated into proteins. In eukaryotes, lncRNAs regulate gene expression at the transcriptional, post-transcriptional, and epigenetic levels. Most research on lncRNAs has been conducted in animals or for medical applications, and lncRNA research in plants is relatively lacking.

Lifeasible, as a leading global company, is committed to helping our customers achieve effective and successful research. We provide analysis services for plant lncRNA verification, including expression, localization, non-coding ability, and interaction validation analyses. In addition, we deliver reliable results and reports on time to our customers worldwide.

Plant lncRNA Expression Validation Analysis

• Long non-coding RNAs (lncRNAs) are a series of transcripts with critical biological functions. After the high-throughput analysis, northern blot, reverse transcription quantitative PCR (RT-qPCR), and others can be used to verify the lncRNAs.
• We offer a variety of plant lncRNA expression validation methods. RT-qPCR is the more commonly used validation method, which is simple and sensitive and can detect the expression level of multiple or single lncRNAs by TaqMan probe or SYBR Green method. Northern blot can directly detect the presence of target lncRNA and the expression pattern before and after or during the treatment and is a classical means with a more tedious experimental procedure.

Fig. 1 Methods for plant lncRNA validation analysis.

Plant lncRNA Localization Validation Analysis

• LncRNAs are involved in biological processes throughout the cell, including the nucleus, chromatin, and cytosol. The localization of lncRNA within the cell is the primary determinant of their molecular functions.
• We help our customers perform plant lncRNA localization validation analysis, including fluorescence in situ hybridization (FISH) and subcellular localization prediction with deep learning.

Plant lncRNA Non-Coding Ability Validation Analysis

• In the process of bioinformatics analysis, although we have used a variety of coding abilities to predict its coding ability, it is best to verify the coding ability in vitro.
• We help our customers analyze plant lncRNA non-coding ability validation, including prediction methods, vector construction, immunofluorescent staining, western blotting, etc.

Plant lncRNA Interaction Validation Analysis

• lncRNAs can bind to RNA, DNA, proteins, etc. The study of lncRNA function can be inferred by studying the molecules interacting with it and the mechanism. We can use antibodies to known proteins to capture RNA molecules or complementary nucleotides of labeled known RNA molecules to capture proteins that interact with them.
• We also offer plant lncRNA interaction validation analysis. We always use RNA-pull-down experiments, RNA-binding protein immunoprecipitation (RIP), chromatin isolation by RNA purification (ChIRP), capture hybridization analysis of RNA targets (CHART), and crosslinking-immunopurification (CLIP) to verify lncRNA targets.

Lifeasible is always devoted to providing high-quality and satisfactory service to our customers. If you are interested in our services or have questions, please contact us or make an online inquiry.

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Related Services

• Identification of Plant lncRNA
• Analysis of Plant lncRNA Structure
• Analysis of Plant lncRNA Functions