Long non-coding RNAs (lncRNAs) are a group of poorly conserved RNA molecules with a sequence length of more than 200 nucleotides and no protein encoding capability. Research on plant lncRNAs is just beginning and has gradually emerged in plant molecular biology. LncRNA sequencing (lncRNA-seq) is a powerful tool to study the functional role in different biological processes, such as plant growth and development and stress response processes.

Lifeasible provides plant lncRNA sequencing services, including inclusive solutions for lncRNA identification, quantification, differential expression, enrichment analysis, and new gene prediction, to help our customers worldwide in plant science research. Our platform is equipped with cutting-edge facilities and professional experts to support research. Here, we provide various services according to customers' demands.

Strand Specific RNA Sequencing

• Transcriptome library construction and sequencing cannot separate the sense and antisense strands, making it difficult to identify and screen many lncRNAs. With the wide application of a strand-specific library, the continuous development of lncRNA prediction algorithms, and the continuous updating of plant genome sketches, many potential lncRNAs have been screened and identified in plants.
• We provide plant lncRNA sequencing services based on strand-specific RNA-seq (ssRNA-seq). There are various approaches to performing ssRNA-Seq; the most used is the dUTP method. Firstly, we prepare samples, including RNA purification and quality assessment; secondly, we synthesize cDNA, the first strand with dNTP and the second strand with dUPT; at last, we perform sequencing analysis and data analysis.
• We used the ssRNA-seq approach to help customers with lncRNA identification in Arabidopsis, kale, cotton, and others. We also visualize and pre-process the results and perform custom bioinformatics analysis.

Third-Generation Sequencing

• Based on the development of third-generation sequencing technology, information regarding lncRNAs on the sense strand and the antisense strand can be obtained by full-length transcriptome sequencing without constructing a strand-specific library. Currently, a large number of lncRNAs have been identified and screened.
• We have helped customers identify and screen many lncRNAs in Arabidopsis, rice, maize, and other species through third-generation sequencing. The general workflow is sample preparation, library preparation, high-fidelity sequencing, and data analysis.
• Our reports and output data files are delivered in industry-standard file formats such as BAM, .xls, .png, and FASTQ data. In addition, we can provide expedited services; please contact us for more details.

Lifeasible provides cost-effective, high-quality, and hassle-free services to our customers worldwide. We provide our clients with direct access to our experts and prompt responses to their questions. If you are interested in our services or have questions, please contact us or make an online inquiry.

*If your organization requires the signing of a confidentiality agreement, please contact us by email.

For research use only, not intended for any clinical use.

Related Services

• Plant lncRNA Microarray
• Bioinformatics Analysis