Plant stem cells include shooting apical meristem (SAM), root apical meristem (RAM), lateral meristem, and some medullary ray cells, which have the ability to produce all differentiated cell types as well as vigorous division ability and high genetic stability. Plant stem cells are characterized by multiple small vesicles, high mitochondrial activity, low aggregation, and no lignin deposition in the cell wall. They maintain high cellular activity after cryopreservation, exist mostly as single cells in suspension culture, are insensitive to shear forces, can maintain a stable proliferation rate, and can be cultured stably for a long time.

What We Offer

• Molecular regulation of SAM stem cells

Lifeasible has extensive experience in the molecular regulation of SAM stem cells, with analysis focusing on WUS-CLV3-centered regulatory pathways, ROS-mediated regulatory pathways, GRAS family, miRNAs, phytohormones, etc.

• Molecular Regulation of RAM Stem Cells

The analysis focused on the WOX5-CLE40-ACR4 regulatory pathway, SHR/SCR regulatory pathway, ROS-mediated regulatory pathway, phytohormones, etc.

• Plant Stem Cell Culture

Lifeasible provides plant stem cell culture services. We have improved our existing methods based on traditional tissue culture techniques. We target plant stem cells, induce, isolate, and culture exosomes, and establish corresponding stem cell culture systems.

With our mature system and rich experience, we accurately control the key points of the plant stem cell culture process to improve the content and yield of target components in dedifferentiated cells. Common methods include cell line preference, optimization of culture conditions, use of inducers, precursor feeding, stage culture, perfusion culture, cell immobilization, etc. And we will choose different methods according to the specific situation to obtain the desired results.

We Do Better

The plant stem cell research platform provided by Lifeasible can be used to discover genes related to the maintenance of plant stem cell populations and stem cell differentiation, and to meet researchers' needs for plant growth and development research by analyzing and tracking the complex regulatory networks of these gene regulatory pathways and exogenous signaling pathways. Plant cells are not contaminated by animal pathogens, the production cost is low, and plant stem cell culture has many advantages, researchers use genetic engineering means to produce antibodies, vaccines, and other protein drugs using plant stem cells obtained from our analysis as receptors, etc. Our customers can use our services as a basis to expand their own research projects, we will always escort you.

Reference

1. James W., et al., "Plant stem-cell organization and differentiation at single-cell resolution." Proceedings of the National Academy of Sciences, 2020, 117(52):33689-33699.

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For research or industrial use.

Related Services

• Plant Phenomics
• Plant Ionomics