Food Allergen Detection

Along with the yearly increase in the occurrence of food allergic diseases, food allergy has now become a more prominent issue in the field of food safety. Food allergens are extremely stable and resistant not only to the action of the digestive tract but also to heat, proteolysis, hydrolysis, and digestion. Allergic reactions can affect the quality of life of patients in mild cases and endanger their lives in severe cases.

Plant Origin Food Allergen Protein

ID	Project	Description
1	Alcohol-soluble proteins	Seed storage proteins
2	Non-specific lipid transfer proteins	Involved in lipid transport, plant defense
3	2S clear proteins	Seed storage proteins
4	Bet v 1 family	Pathogenesis-related proteins
5	7S globulin	Seed storage proteins
6	11S globulin	Seed storage proteins
7	Cysteine protease C1 family	Cysteine proteases
8	Inhibitor protein	Actin-binding proteins

Our Solution

Mass spectrometry-based food allergen detection

We use unique protein extraction and purification methods in our sample pre-treatment methods to improve extraction and purification efficiency. Alternatively, we use ultrasound-assisted enzymatic digestion or multiple enzymes acting simultaneously to speed up enzymatic digestion. In the detection, according to different purposes Different mass spectrometry detection techniques, different internal standards, and quantitative methods are selected.

• MALDI-TOF MS

• UPLC-MS/MS
• Peptide mass fingerprinting (PMF)
• Peptide fragment ion identification (PFI)
• MRM method for quantitative analysis
• QqQ-MS
• Q-LIT-MS
• Q-TOF-MS
• Orbitrap-MS
• Q-Orbitrap-MS
• LTQ-Orbitrap-MS
• Orbitrap Fusion-MS

Food allergen detection based on immunological techniques

Immunological detection technology is a detection technology based on antigen-antibody-specific reactions. This technique is characterized by high sensitivity and specificity and is capable of rapid and accurate qualitative and quantitative detection of antigenic substances.

• Isoelectric focusing (IEF) and SDS-PAGE electrophoresis

• Radio allergo sorbent test (RAST)
• Enzyme allergo sorbent test (EAST)
• Fluorescent and chemiluminescent labeling
• Rocket immunoelectrophoresis (RIE)
• Lateral-flow immunochromatography assay (LFA)
• Time-resolved fluorescence assay (TRFIA)
• Immunosensor assay technology
• Immunochromatography assay (ICA)
• Dot immunoblotting
• ELISA

PCR-based food allergen detection

• Real-time fluorescence quantitative PCR
• Loop-mediated isothermal amplification (LAMP)

Biochip Protein microarray technology Biosensing technology

• Optical biosensors
• Electrochemical biosensors
• Piezoelectric Immunosensors

Key Advantages

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For research or industrial use.

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