PRODUCT
Chloroplast Protein Extraction Kit (For Proteomic Assay and Mass Spectrometry)-Enzymic Method
| Cat# | EK-013 |
| Specification | 50T, 100T |
| Unit | Box |
| Description | The Chloroplast Protein Extraction Kit (For Proteomic Assay and Mass Spectrometry) is a simple and fast method to extract the total chloroplast protein from various plant samples. This kit is suitable for extracting chloroplast proteins from fresh plant samples, but the recovery of chloroplast proteins from frozen samples is low due to the fact that most of the chloroplasts are destroyed during the freezing process. |
| Storage Conditions | Store at 2-8℃. |
| Shelf Life | 12 months |
| Applicable Samples | Plant |
| Applications | The proteins extracted in this kit can be used in downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation, ELISA, transcriptional activity analysis, Gelshift gel block assay, enzyme activity assay and so on. |
| Features | This kit contains a unique formulation that can effectively solubilize chloroplast membrane components. This kit contains a mixture of protease inhibitors that prevents the degradation of proteins by proteases, providing for the extraction of high purity proteins. The proteins extracted in this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation chromatography. |
| Advantages | This kit uses enzymatic extraction, and chloroplasts prepared by enzymatic extraction have increased recovery and high purity, but are time consuming. Non-enzymatic extraction kit extraction process is simple and convenient, fast, can be completed in 1 hour, and never cross contamination, but the recovery rate is lower compared to the enzymatic method. |
| Precautions | If you need a faster extraction kit, you can choose the non-enzymatic extraction kit, and if you don't need the extraction speed, you can choose the enzymatic extraction kit. Please choose the kit according to the actual need. |
