PRODUCT
GUS Stain Kit
| Cat# | PSR-001 |
| Specification | 100 mL |
| Unit | Box |
| Description | The GUS (β-Galactosidase) gene is a hydrolase encoding β-glucuronidase that exists in the genomes of some bacteria such as E. coli. This gene is often used as a fusion marker in plant transgenic analysis and regulation research. The GUS receptor gene system expresses the stability of E.coli GUS enzyme and has low activity in plants. Most plants have no detectable background activity of glucuronidase and use β-glucuronide esters as substrates. , the reaction products can be detected by a variety of methods. 5-Bromo-chloro-3-indolyl β-D-glucuronide, cyclohexylammonium salt (referred to as X-Gluc or X-GlcA) is a substrate for detecting GUS genes in E. coli and can quickly detect GUS gene fusion markers in plants. |
| Storage Conditions | Store at -20℃, protected from light. |
| Shelf Life | 6 months |
| Detection Principle | GUS Stain Kit is the abbreviation of β-Galactosidase Reporter Gene Staining Kit. The principle of GUS staining solution is that under appropriate reaction conditions, β-glucosidase (GUS) can hydrolyze X-Gluc into a blue substance. This substance is insoluble in the indigo substance in the genetically modified cell nucleus tissue and has GUS activity. Or the site appears blue or blue spots, which can be observed with the naked eye or microscope. |
| Applications | Can be used for biochemical activity analysis, immunoassay, and histochemical staining of tissues and cells. It is mostly used for GUS gene expression analysis of transgenic plants. |
