Micro Plant Anthocyanin Assay Kit

Cat#

PBK-015

Specification

100T/48S

Unit

Box

Description

Chromogranins are a class of natural pigments that are soluble in polar solvents and belong to the flavonoids. Chromogranin is widely found in the roots, stems, leaves, flowers and fruits of plants, making them show different colors from red to purple, and it is the main color-presenting substance of plants.

Test Method

Spectrophotometer/Microplate Reader

Storage Conditions

2-8℃

Shelf Life

6 months

Detection Principle

The pH-differential method is used to determine the content of anthocyanin, when the pH is 1.0, anthocyanin has the maximum absorption peak at 530 nm, and when the pH is 4.5, anthocyanin is transformed into a colorless chalcone form, and there is no absorption peak at 530 nm, so we can use this characteristic to determine the absorbance values at 530 nm and 700 nm under different pH. pH-differential method reduces the effects of the difference of the pH of solution and the solvent, and excludes other non-anthocyanin substances. The pH-differential method reduces the influence of solution pH and solvent differences, and excludes the interference of other non-anthocyanidin substances on the results.

Self-prepared Laboratory Supplies and Equipment

Visible spectrophotometer/enzymometer, water bath, adjustable pipette, micro-quartz cuvette/96-well plate, mortar and distilled water.