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pG-Tn5 Transposome

Cat# PEs-003
Description pG-Tn5 transposase fuses Protein G with a highly active, mutant form of Tn5 transposase, which functions as both a Tn5 transposase and Protein G. pG-Tn5 transposase is a highly active, mutant form of Tn5 transposase, which functions as a Tn5 transposase. This product fuses a mutant form of Tn5 transposase, which is 1000-fold more efficient than wild-type transposition in vitro. This product is a pG-Tn5 transposase complex with completed junction assembly, which can be used for DNA sample fragmentation, CUT&Tag.
Molecular Weight 70 kD
Source The pG-Tn5 transposase protein was recombinantly expressed in E. coli and obtained by purification.
Characteristic One-step DNA fragmentation and junction connection. Protein purity >95% by SDS-PAGE, no nuclease contamination. High DNA fragmentation efficiency with both Protein G & Tn5 activities.
QC No DNase, RNase activity. No exonuclease or endonuclease activity. No microbial genome residue by PCR. Protein purity >95% by SDS-PAGE.
Specification 12 μL, 48 μL
Content 4 μM
Application Second-generation sequencing library construction ATAC-seq CUT&Tag
Storage Store at -20°C