PRODUCT
Plant ATP Synthase ELISA Kit
| Cat# | PEK-065 |
| Specification | 96T/48T |
| Target | Plant ATP Synthase |
| Description | The ELISA kit applies the double-antibody sandwich method to determine the level of Plant ATP Synthasein plant samples. Purified Plant ATP Synthaseantibodies are coated onto a microplate to form a solid-phase antibody. To these coated monoclonal antibody wells, Plant ATP Synthaseis added, followed by an HRP-conjugated Plant ATP Synthaseantibody, forming an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added and turns blue under the catalysis of HRP, and then changes to a final yellow color due to the addition of acid. The color intensity is directly proportional to the amount of Plant ATP Synthasepresent in the sample. The absorbance (OD value) is measured at 450 nm using an ELISA reader, and the concentration of Plant ATP Synthasein the plant samples is calculated based on a standard curve. |
| Application | This kit is used to quantitatively determine the content of Plant ATP Synthase in plant samples. |
| Composition | Instructions Sealing Membrane Seal Bag Coated Plate Standard Standard Diluent Enzyme Conjugate Reagent Sample Diluent Chromogen A Solution Chromogen B Solution Stop Solution Concentrated Wash Solution |
| Note | Samples containing NaN3 cannot be tested because NaN3 inhibits the activity of horseradish peroxidase (HRP). |
| Sample Handling and Requirements | 1. The sample must be kept fresh 2. The sample weight should not be less than 50mg, usually 1g is used as the benchmark, but it is not strictly required that each weight of the fixed sample must reach the same level. 3. The proportion of homogenization is 10%, which is equivalent to 1g tissue plus 9ml homogenization solution for homogenization. 4. The homogenization solution is PBS (PH=7.2-7.4, concentration is 0.01mol/L) 5. The homogenization process uses a tissue homogenizer and homogenizes on an ice bath. (Or liquid nitrogen grinding) 6. Centrifuge to obtain the supernatant, the centrifugal speed is 5000 rpm, and the time is 15 minutes. Take the supernatant for inspection! |
| Operation Step | 1. Standard spiking: Add 50 μL standards to well. 2. Sample addition: Add 40 μL of sample dilution to the sample wells on the enzyme plate, and then add 10 μL of the sample to be tested (the final dilution of the sample is 5 times), and mix well. 3. Add enzyme and warming: Add 100 μL of enzyme reagent to each well, except for blank wells, 37℃ for 30 minutes. 4. Washing: Discard the liquid, add washing solution, 30 seconds and discard, repeat 5 times. 5. Color development: Add 50 μL CA and then 50 μL CB and mix, 37℃ for 15 minutes at avoiding light. 6. Determination: Measure the OD after the addition of the termination solution. |
| Storage Conditions | 2-8℃ |
| Shelf Life | 6 months |
