PRODUCT
Plant Leaf Direct PCR Plus Kit
| Cat# | EK-033 |
| Specification | 200T |
| Unit | Box |
| Description | This product utilizes a unique lysis buffer system that enables rapid release of genomic DNA from leaf samples of plants with high polysaccharide and polyphenol content (e.g., cotton, banana, etc.) for PCR reactions. |
| Storage Conditions | Part I stored at 2-8°C; Part II stored at -20°C |
| Shelf Life | Reagents Buffer PS1, Buffer P2, 6× DNA Loading Buffer can be stored for 12 months under dry conditions; if you need to store for a longer period of time, it can be stored at 2-8℃. Reagent Buffer PS2, 2× Leaf PCR Mix, can be stored at -20℃ for 12 months; if used frequently, it can be stored at 4℃ for a short period of time (limited to be used up within 10 days). |
| Applications | Scope of application: a wide range of plant leaves. DNA released from sample cleavage: used as PCR template only. The kit can be used for the following purposes: transgenic plant identification, plant genotyping, etc. |
| Product Composition | Buffer PS1: Provides the necessary environment for plant leaf lysis reaction. Buffer PS2: Supplemental to provide the environment for the lysis reaction of polysaccharide and polyphenol plant leaves. Buffer P2: Neutralize the lysis products so that they do not affect the subsequent PCR reaction. 2× Leaf PCR Mix: Containing Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR enhancement agent, optimizer and stabilizer, etc. For PCR reaction, just add the appropriate lysis mix, primer and ddH2O into the 2× Leaf PCR Mix and then it can be used for PCR reaction. 6× DNA Loading Buffer: The Loading Buffer does not contain SDS, and it is recommended to use the 6× DNA Loading Buffer that comes with the kit for agarose gel electrophoresis in order to obtain good electrophoresis results. |
| Features | The lysis buffer treats leaves without the need for grinding or shearing of the leaves, making it particularly suitable for large-scale genetic testing. The process of releasing genomic DNA from the lysis buffer can be completed within 5-10 min without other processes of removing proteins, RNA or secondary metabolites, and the released trace DNA can be used as a template for PCR reaction. |
| Advantages | Eliminates the need for time-consuming and expensive DNA purification. Small sample size, 2 mm (1 mg) diameter leaves can be used for experiments. No special handling such as grinding and crushing of leaves, easy to operate. Optimized PCR system for higher specificity and better tolerance of PCR inhibitors. |
| Precautions | 1. Pay attention to the cleanliness of the experimental tools and the experimental practices to avoid cross contamination between samples. 2. Please try to use fresh and young leaves for the experiment. If mature leaves are used, please avoid using tissues from the main veins of the leaves. If precipitation occurs from Buffer PS1, leave it at 37℃ until the precipitation disappears and mix the solution well before use. 3. 2× Leaf PCR Mix should avoid repeated freezing and thawing, otherwise the PCR efficiency will be affected. 4. If the ambient temperature is too high, the 2× Leaf PCR Mix may become turbid, so it can be placed on ice for 1-2min, wait until the solution is clarified, and mix the solution upside down for 3-5 times before use. 5. Do not use SDS-containing Loading Buffer during electrophoresis, otherwise a large trailing bright band will appear in the lane, which will affect the results of the experiment. |
