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PRODUCT

Plant Protein Extraction Kit (For Proteomics Experiments)

Cat# EK-002
Specification 50T, 100T
Unit Box
Description Plant Protein Extraction Kit (For Proteomics Experiments) is suitable for extracting total protein from various fresh plant tissue samples. The extraction process is simple and convenient, and can be completed within 1 hour.
Storage Conditions Store at 2-8℃.
Shelf Life 12 months
Applicable Samples Plant
Applications The extracted proteins in this kit can be used in protein profiling, WesternBlot, protein electrophoresis, 2-D, IEF, Pull-down, co-IP, IP, ELISA, transcriptional activity analysis, Gelshift gel blocking assay, enzyme activity assay and other downstream protein research experiments.
Features The kit contains a unique formulation that effectively solubilizes cell membrane components, including the plasma membrane, nuclear membrane, and various organelle membranes. The protease inhibitor mixture contained in this kit prevents protein degradation by proteases, providing for the extraction of high purity proteins. The protein extraction components of this kit do not contain descaler components that cannot be removed by dialysis, and do not contain SDS, TritonX-100, chaps and other components that may affect mass spectrometry experiments, and the protein samples obtained at the end of the dialysis or desalination will not contain descaler, high concentration of salt and other influences, which basically satisfy the downstream of any proteomics-related experimental research. The protease inhibitor mixture of this product does not contain AEBSF, which can avoid the drift of mass spectrometry peaks caused by AEBSF, so the protein samples extracted by this product can be used for mass spectrometry (MS) detection and analysis, proteomics and other related research. The proteins extracted in this kit are active proteins with natural protein conformation. This kit does not contain EDTA and is compatible with downstream applications such as metal chelation chromatography.