Cas12 ssDNA Reporter (FAM/BHQ)

Cat#

CASR-003

Introduction

Cas12 ssDNA reporter (FAM/BHQ) is a single-strand oligo DNA probe with a 6-FAM (fluorescence) label at the 5' end and a BHQ (quencher) label at the 3' end. It is designed to be used in CRISPR-Cas12 activity assays. When combined with guide RNA and target DNA, Cas12a, Cas12b, and Cas12f enzymes exhibit activated trans-cleavage activity against ssDNA. Upon cleavage, the quenched fluorescence of the probe recovers, enabling enzyme activity analysis and target detection

Size

10 nmol/Bulk Customize

Overview

Store at room temperature (15-30°C), in a dry, light-protected environment. After reconstitution, store the stock solution (100 μM) at -80°C in the dark for 6 to 12 months without repeated freeze-thaw cycles. The working solution (10 μM) can be stored at -20°C in the dark and used within 3 to 6 months

Application

Protein is white to off-white lyophilized powder in dark tube