PRODUCT
EiCsm6
Cat#
CASPT-004
Source Organism
Enterococcus italicus
Expression Host Organism
E. coli
Tag Type
His
Sequence
full length
Introduction
Recombinant full-length EiCsm6 from Enterococcus italicus was expressed in E. coli using a C-terminal His tag.
Size
50 ug/100 ug/Bulk Customize
Alternate Names
Enteroccocus italicus Csm6, CRISPR type III-A associated protein Csm6
UniProt Number
E6LHV2
Overview
EiCsm6 is an ancillary ribonuclease associated with the type III CRISPR-Cas system of Enterococcus italicus. Like other Csm6 family proteins, EiCsm6 consists of a CARF (CRISPR-associated Rossman fold) domain, which binds cyclic oligoadenylate (cOA), and a HEPN (Higher Eukaryotes and Prokaryotes Nucleotide binding) domain, which possesses ribonuclease activity (1). The RNA degradation activity of EiCsm6 is stimulated by cOA and functions to protect the bacterial cell from invasive genetic elements like bacteriophages and plasmids (2, 3).
Accession Number
WP_007208953.1
Formulation
Protein stored in 50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 200 mM imidazole, 1 mM DTT, 10% glycerol
References
1. Niewoehner O, Jinek M. 2016. Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. RNA. 22(3):318-29.
2. Rostøl, J.T., Marraffini, L.A. 2019. Non-specific degradation of transcripts promotes plasmid clearance during type III-A CRISPR–Cas immunity. Nat Microbiol 4:656–662.
3. Niewoehner O, Garcia-Doval C, Rostøl JT, Berk C, Schwede F, Bigler L, Hall J, Marraffini LA, Jinek M. 2017. Type III CRISPR-Cas systems produce cyclic oligoadenylate second messengers. Nature. 548(7669):543-548.
2. Rostøl, J.T., Marraffini, L.A. 2019. Non-specific degradation of transcripts promotes plasmid clearance during type III-A CRISPR–Cas immunity. Nat Microbiol 4:656–662.
3. Niewoehner O, Garcia-Doval C, Rostøl JT, Berk C, Schwede F, Bigler L, Hall J, Marraffini LA, Jinek M. 2017. Type III CRISPR-Cas systems produce cyclic oligoadenylate second messengers. Nature. 548(7669):543-548.
Storage and Stability
Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities.
Concentration
0.5 μg/μl
19.38 pmol/μg
19.38 pmol/μg
Purity
>95%
Molecular weight
Calculated: 51.9 kDa
Observed: ~50 kDa
Observed: ~50 kDa
Recommendation
Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.
