PRODUCT
FnCas12a, Glycerol-Free
Cat#
CASPT-006
Source Organism
Francisella tularensis
Expression Host Organism
E. coli
Tag Type
His
Sequence
full length
Introduction
Recombinant His-tagged Francisella tularensis protein expressed in E. coli. Enzyme supplied with 1 mL of Cas12 reaction buffer, 10x (CS12-09).
Size
50 ug/100 ug/Bulk Customize
Alternate Names
CRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1 1, FnCas12a 1, FnCpf1
UniProt Number
A0Q7Q2
Overview
CRISPR (clustered regularly interspaced short palindromic repeats) and CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for RNA-based therapeutics development (3). Cas12 and Cas13 have collateral nucleotide cleavage activities and are widely used in CRISPR-based diagnostic platforms. FnCas12a from Francisella tularensis subsp. novicida shows increased stability at >37°C, making it ideal for LAMP (loop-mediated isothermal amplification) applications (4).
Accession Number
WP_003040289
Formulation
Protein stored in 25 mM Tris, 300 mM NaCl, pH7.5, 0.1 mM TCEP
References
1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
4. Mohanraju P, et al: Development of a Cas12a-Based Genome Editing Tool for Moderate Thermophiles. CRISPR J. Feb;4(1):82-91. 2021.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
4. Mohanraju P, et al: Development of a Cas12a-Based Genome Editing Tool for Moderate Thermophiles. CRISPR J. Feb;4(1):82-91. 2021.
Storage and Stability
Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities.
Concentration
1.0 μg/μl
6.53 pmol/μg
6.53 pmol/μg
Purity
>90%
Molecular weight
Calculated: 153.2 kDa
Observed: ~130 kDa
Observed: ~130 kDa
Recommendation
Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.
