PRODUCT
LbCas12a, GST-Tagged
Cat#
CASPT-007
Source Organism
Lachnospiraceae
Expression Host Organism
E. coli
Tag Type
GST
Sequence
full length
Introduction
Recombinant Lachnospiraceae LbCas12a (LbCpf1) (1-1227) was expressed in E. coli with an N-terminal GST tag. Enzyme supplied with 1 mL of Cas12 reaction buffer, 10x (CS12-09).
Size
50 ug/100 ug/Bulk Customize
Alternate Names
Cpf1 (synthetic construct)
UniProt Number
A0A5S8WF58
Overview
CRISPR (clustered regularly interspaced short palindromic repeats) and their CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria (1-2). This system has been redesigned to create an exemplary genome editing tool for application to RNA-based therapeutics development (3) and disease diagnosis of viral pathogens. Cas12 and Cas13 are recognized as ideal candidates for future development and management of diseases such as cancer (4). Several CRISPR-Cas12-based SARS-CoV-2 detection assays have been developed (5). CRISPR/Cas12 (LbCpf1) is a Cas12 synthetic construct derived from Lachnospiraceae bacteria.
Accession Number
ATB19154.1
Formulation
Protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 1 mM DTT, 10% glycerol
References
1. Horvath, P., et al. 2010. CRISPR/Cas, the immune system of bacteria and archaea. Science, 327(5962):167-170.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
4. Singh, M., et al. 2022. The era of Cas12 and Cas13 CRISPR-based disease diagnosis. Critical Reviews in Microbiology, DOI: 10.1080/1040841X.2021.2025041.
5. Broughton JP, et al: Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR-based DETECTR lateral flow assay. medRxiv doi: https://doi.org/10.1101/ 2020.03.06.20032334.
2. Morange, M. 2015. What history tells us XXXVII. CRISPR-Cas: The discovery of an immune system in prokaryotes. J. Biosci. 40(2):221-223.
3. Gier, R.A., et al. 2020. High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening. Nat. Commun. 11(1):1-9.
4. Singh, M., et al. 2022. The era of Cas12 and Cas13 CRISPR-based disease diagnosis. Critical Reviews in Microbiology, DOI: 10.1080/1040841X.2021.2025041.
5. Broughton JP, et al: Rapid detection of 2019 novel coronavirus SARS-CoV-2 using a CRISPR-based DETECTR lateral flow assay. medRxiv doi: https://doi.org/10.1101/ 2020.03.06.20032334.
Storage and Stability
Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities.
Concentration
0.1 μg/μl
5.88 pmol/μg
5.88 pmol/μg
Purity
>70%
Molecular weight
Calculated: 171 kDa
Observed: ~150 kDa
Observed: ~150 kDa
Recommendation
Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.
