PRODUCT
LsCsm6
Cat#
CASPT-011
Source Organism
Ligilactobacillus salivarius
Expression Host Organism
E. coli
Tag Type
His
Sequence
full length
Introduction
Recombinant Ligilactobacillus salivarius protein LsCsm6 was expressed in E. coli cells using a His tag.
Size
50 ug/100 ug/Bulk Customize
Alternate Names
CRISPR system endoribronuclease Csm6, CRISPR type III-A associated protein Csm6
Overview
LsCsm6 is a single-stranded RNA-specific endoribonuclease that consists of the N-terminal CARF domain and the C-terminal HEPN domain. The ribonuclease active site is formed by dimerization of the HEPN domains. LsCsm6 belongs to the Class 1 type-III-A CRISPR-Cas system (1). Upon activation, the CRISPR ancillary ribonucleases Csx1/Csm6, the HEPN active site degrades invasive RNA in a non-specific manner. LsCsm6 is activated by cyclic oligoadenylate second messengers synthesized from ATP by the Cas10 subunit of the Type III interference complex (2).
Accession Number
WP_081509150
Formulation
Protein stored in 25 mM Tris, 300 mM NaCl, pH 7.5, 0.1 mM TCEP, 50% glycerol
References
1. Niewoehner, O. et al. 2016. Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. RNA 22(3):318-329.
2. Niewoehner, O. et al. 2017. Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548.
2. Niewoehner, O. et al. 2017. Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548.
Storage and Stability
Store at –20°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities.
Concentration
1.0 μg/μl
20.4 pmol/μg
20.4 pmol/μg
Purity
The purity was determined to be >90% by densitometry
Molecular weight
Calculated: 49.6 kDa
Observed: ~49 kDa
Observed: ~49 kDa
Recommendation
Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.
