PRODUCT
LwCas13a
Cat#
CASPT-012
Source Organism
Leptotrichia wadei
Expression Host Organism
E. coli
Tag Type
His
Sequence
full length
Introduction
Recombinant full-length, His-tagged Leptotrichia wadei protein LwCas13a expressed in E. coli. Enzyme supplied with 1 mL of Cas13 reaction buffer II, 10x (CS13-09B).
Size
50 ug/100 ug/Bulk Customize
Alternate Names
LwC2c2, EndoRNase, CRISPR-associated endoribonuclease Cas13a
UniProt Number
U2PWF1
Overview
Cas13a is an RNA-guided RNA endonuclease that belongs to the Class 2 type VI CRISPR-Cas system (1). Cas13a possesses both RNA recognition and cleavage activities and cleaves RNA through conserved basic residues within its two HEPN domains (2). Activated Cas13a also induces collateral cleavage of nearby non-targeted RNAs in a nonspecific manner (3). This property of the Cas13a enzyme has been leveraged for the development of sensitive and robust nucleic acid detection tools with applications in both basic research and therapeutics development (4).
Accession Number
WP_021746774.1
Formulation
Protein stored in 25 mM Tris, 300 mM NaCl, pH 7.5, 0.1 mM TCEP, 50% glycerol
References
1. East-Seletsky A, et al. 2016. Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. Nature. 538:270–3. 2.
2. Abudayyeh O, et al. 2016. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 353:5573 3.
3. Zhang J, You Y. 2020. CRISPR-Cas13a system: A novel approach to precision oncology. Cancer Biol Med. 17(1):6-8.
4. Patchsung M, et al. 2020. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 4(12):1140-1149.
2. Abudayyeh O, et al. 2016. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science. 353:5573 3.
3. Zhang J, You Y. 2020. CRISPR-Cas13a system: A novel approach to precision oncology. Cancer Biol Med. 17(1):6-8.
4. Patchsung M, et al. 2020. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 4(12):1140-1149.
Storage and Stability
Store at –20°C. To avoid repeated handling and multiple freeze/thaw cycles, aliquot product into smaller quantities.
Concentration
1 μg/μl
7.14 pmol/μg
7.14 pmol/μg
Purity
>90%
Molecular weight
Calculated: 139.8 kDa
Observed: ~125 kDa
Observed: ~125 kDa
Recommendation
Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.
