PRODUCT
Micro Plant Dehydrogenase(PDHA) Activity Assay Kit
Cat# | PBK-023 |
Specification | 100T/48S |
Unit | Box |
Description | The activity of Plant dehydrogenase (DHA) reflects to a large extent the activity state of the organism, and can directly indicate the strength of the degradation ability of the biological cell to its substrate. |
Test Method | Spectrophotometer/Microplate Reader |
Storage Conditions | 2-8℃ |
Shelf Life | 6 months |
Detection Principle | After the hydrogen acceptor 2,3,5-Triphenyl Tetrazolium Chloride (TTC) receives hydrogen in the process of cellular respiration, its reduction product Triphenyl Formazone (TF) shows red color, and it has the maximum absorption peak at the wavelength of 485 nm, which is measured at 485 nm. The maximum absorption peak is at 485nm, and its absorbance value at 485nm is measured to obtain the activity of plant dehydrogenase. |
Self-prepared Laboratory Supplies and Equipment | Thermostatic incubator or water bath, low-temperature centrifuge, mortar and pestle, filter paper, visible spectrophotometer/enzymometer, micro-quartz cuvette/96-well plate, ice, distilled water and ethyl acetate. |