PRODUCT
pA-Tn5 Transposome
| Cat# | PEs-002 |
| Description | pA-Tn5 transposase fuses Protein A with a highly active, mutant form of Tn5 transposase, which functions as both a Tn5 transposase and Protein A. pA-Tn5 transposase is a highly active, mutant form of Tn5 transposase. The fusion is a mutant form of Tn5 transposase, which is 1000-fold more efficient than wild-type transposition in vitro. This product is a pA-Tn5 transposase complex with completed junction assembly, which can be used for DNA sample fragmentation, CUT&Tag. |
| Molecular Weight | 70 kD |
| Source | The pA-Tn5 transposase protein was recombinantly expressed in E. coli and obtained by purification. |
| Characteristic | One-step DNA fragmentation and junction connection. Protein purity >95% by SDS-PAGE, no nuclease contamination. High DNA fragmentation efficiency with both Protein A & Tn5 activities. |
| QC | No DNase, RNase activity. No exonuclease or endonuclease activity. No microbial genome residue by PCR. Protein purity >95% by SDS-PAGE. |
| Specification | 12 μL, 48 μL |
| Content | 4 μM |
| Application | Second-generation sequencing library construction ATAC-seq CUT&Tag |
| Storage | Store at -20°C |
