PRODUCT
pAG-Tn5 Transposase for CUT&Tag
| Cat# | PEs-004 |
| Description | pAG-Tn5 Transposase fuses Protein A/Protein G with a highly active, mutant form of Tn5 transposase, which functions as both a Tn5 transposase and a Protein A/Protein G. This product is a highly active, mutant form of Tn5 transposase, which is 1000-fold more efficient than wild-type transposase in vitro. This product is fused with a mutant form of Tn5 transposase, which is 1000-fold more efficient than wild-type transposition in vitro. This product contains pAG-Tn5 transposase and related assembly buffer and is specifically suited for CUT&Tag technology for protein-genome interaction studies. |
| Molecular Weight | 75 kD |
| Source | The pAG-Tn5 transposase gene was recombinantly expressed in E. coli and purified and obtained. |
| Characteristic | One-step DNA fragmentation and junction connection. Protein purity >95% by SDS-PAGE, no nuclease contamination. High DNA fragmentation efficiency with both Protein A/G & Tn5 activities. |
| QC | No DNase, RNase activity. No exonuclease or endonuclease activity. No microbial genome residue by PCR. Protein purity >95% by SDS-PAGE. |
| Specification | 20 μg, 100 μg |
| Content | 2 mg/mL |
| Application | Second-generation sequencing library construction ATAC-seq CUT&Tag |
| Storage | Store at -20°C |
