PRODUCT
Plant Leaf Direct PCR Kit
| Cat# | EK-030 |
| Specification | 200T |
| Unit | Box |
| Description | This product utilizes a unique lysis buffer system to rapidly release genomic DNA from leaf samples of plants with low polysaccharide and polyphenol content (e.g., rice, wheat, tobacco, maize, soybeans, oilseed rape, etc.) for use in PCR reactions. |
| Storage Conditions | Part I stored at 2-8°C; Part II stored at -20°C |
| Shelf Life | Reagents Buffer P1, Buffer P2, 6× DNA Loading Buffer can be stored for 12 months under dry conditions; if you need to store for a longer period of time, it can be stored at 2-8℃. Reagent 2× Leaf PCR Mix can be stored at -20℃ for 12 months; if used frequently, it can also be stored at 4℃ for a short period of time (limited to be used within 10 days). |
| Applications | Scope of application: a wide range of plant leaves. DNA released from sample cleavage: used as PCR template only. The kit can be used for the following purposes: transgenic plant identification, plant genotyping, etc. |
| Product Composition | Buffer P1: Provides the environment required for the plant leaf lysis reaction. Buffer P2: neutralize the lysis products so that they do not affect the subsequent PCR reaction. 2× Leaf PCR Mix: contains Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR enhancement agent, optimizer and stabilizer, etc. For PCR reaction, just add the appropriate lysis mixture, primer and ddH2O into the 2× Leaf PCR Mix and then it can be used for PCR reaction. 6× DNA Loading Buffer: The Loading Buffer does not contain SDS, and it is recommended to use the 6× DNA Loading Buffer that comes with the kit for agarose gel electrophoresis in order to obtain good electrophoresis results. |
| Features | The lysis buffer treats the leaves without the need for grinding or shearing of the leaves, making them particularly suitable for large-scale genetic testing. The process of releasing genomic DNA from the lysis buffer can be completed within 5-10 min, without the need for other processes to remove proteins, RNA or secondary metabolites, and the released trace DNA can be used as a template for PCR reactions. |
| Advantages | Eliminates the need for time-consuming and expensive DNA purification. Small sample size, 2 mm (1 mg) diameter blades are sufficient for experiments. No special handling such as grinding and crushing of leaves is required, making it easy to use. Optimized PCR system for higher specificity and tolerance of PCR inhibitors. |
| Precautions | 1. Pay attention to the cleanliness of the experimental equipment and experimental practices to avoid cross contamination between samples. 2. Try to use seeds that are less than 1 year old for the experiment. If the seeds have been stored for more than 1 year or if the seeds are particularly dry, please break the seed coat or use cracked seeds when lysing. 3. If precipitation occurs with Buffer SSP1, leave it at 37°C until the precipitation disappears and shake the solution well before use. 4. Buffer SSP2 should not be exposed to air for a long period of time and should be stored at -20°C after use. If used frequently, it can be stored at 4℃ for a short period of time (within 10 days). 5. 2× Seed PCR Mix should avoid repeated freezing and thawing, otherwise the PCR efficiency will be affected. 6. If the ambient temperature is too high, the 2× Seed PCR Mix may become turbid, so it can be placed on ice for 1-2min, wait until the solution is clarified, and then mix it upside down for 3-5 times before use. 7. Do not use SDS-containing Loading Buffer during electrophoresis, otherwise a large trailing bright band will appear in the lane, which will affect the results of the experiment. |
