Plant Seed Direct PCR Plus Kit I - Plant Tissue Culture

Cat#	EK-031
Specification	200T
Unit	Box
Description	This product adopts a unique cleavage reaction system, which can rapidly release genomic DNA from plant seed samples with high polysaccharide and polyphenol content (e.g. banana, cotton, etc.) for PCR reaction, so it is especially suitable for large-scale genetic testing.
Storage Conditions	Part I stored at 2-8°C; Part II stored at -20°C
Shelf Life	Reagents Buffer SSP1, Buffer SP2, 6× DNA Loading Buffer, can be stored for 12 months under dry condition; if need to be stored for a longer period of time, it can be stored at 2-8℃. Reagent 2× Seed PCR Mix, Buffer SSP2; if used frequently, it can also be stored at 4℃ for a short period of time (use up within 10 days).
Applications	Scope of application: a wide range of plant seeds. DNA released from sample lysis: only used as PCR template. The kit can be used for the following purposes: transgenic seed identification, plant genotyping, etc.
Product Composition	Buffer SSP1: Provides the necessary environment for the lysis reaction of polysaccharide and polyphenol plant seeds. Buffer SSP2: Supplemental to provide the environment required for the lysis reaction of polysaccharide polyphenol plant seeds. Buffer SP2: Neutralize the lysis products so that they do not affect the subsequent PCR reaction. 2× Seed PCR Mix: Containing Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR enhancement agent, optimizer and stabilizer, etc. For PCR reaction, just add the appropriate lysis mix, primer and ddH2O into 2× Leaf PCR Mix and then it can be used for PCR reaction. 6× DNA Loading Buffer: The Loading Buffer does not contain SDS, and it is recommended to use the 6× DNA Loading Buffer included in the kit for agarose gel electrophoresis in order to obtain good electrophoresis results.
Features	In order to meet the needs of different detection experiments, this kit can be used for experiments with various types of samples (e.g., intact seeds, microtissue cuttings, or ground samples of multiple seeds). Among them, for seed samples that still need to undergo germination, one can choose to cut trace tissue cut pieces (1-5 mg) other than seed embryos for experiments; for experiments that need to sample and detect in a large number of samples, one can choose to mix and grind multiple samples into particles with a diameter of about 0.5 mm and then carry out the experiments (a minimum of 0.1% of the target samples can be detected). The process of releasing genomic DNA from the lysis reaction system can be completed within 5-10min, without the need for other processes to remove proteins, RNA or secondary metabolites, and the released trace DNA can be used as a template for PCR reactions.
Advantages	Eliminates the need for time-consuming and expensive DNA purification. Small sample size, only a single seed is required. No special treatment such as grinding and crushing is required, making it easy to operate. Optimized PCR system for higher specificity and better tolerance of PCR inhibitors.
Precautions	1. Pay attention to the cleanliness of the experimental tools and the experimental practices to avoid cross contamination between samples. 2. Please try to use the seeds within 1 year for the experiment. If the seeds have been stored for more than 1 year or the seeds are particularly dry, please break the seed coat or use cracked seeds when lysing. 3. If precipitation occurs in Buffer SSP1, leave at 37°C until the precipitation disappears and shake the solution well before use. 4. Buffer SSP2 should not be exposed to air for a long period of time and should be stored at -20°C after use. If used frequently, it can be stored at 4℃ for a short period of time (within 10 days). 5. 2× Seed PCR Mix should avoid repeated freezing and thawing, otherwise the PCR efficiency will be affected. 6. If the ambient temperature is too high, the 2× Seed PCR Mix may become turbid, so it can be placed on ice for 1-2min, wait until the solution is clarified, and then mix it upside down for 3-5 times before use. 7. Do not use SDS-containing Loading Buffer during electrophoresis, otherwise a large trailing bright band will appear in the lane, which will affect the results of the experiment.