Plant Seed Direct PCR Plus Kit II - Plant Tissue Culture

Cat#	EK-032
Specification	200T
Unit	Box
Description	This product adopts a unique cleavage reaction system, which can rapidly release genomic DNA from plant seed samples with low polysaccharide and polyphenol content (e.g., rice, wheat, tobacco, corn, soybean, etc.) for PCR reaction, and thus is particularly suitable for large-scale genetic testing.
Storage Conditions	Part I stored at 2-8°C; Part II stored at -20°C
Shelf Life	Reagents Buffer SSP1, Buffer SP2, 6× DNA Loading Buffer, can be stored for 12 months under dry condition; if need to be stored for a longer period of time, it can be stored at 2-8℃. Reagent 2× Seed PCR Mix, Buffer SSP2; if used frequently, it can also be stored at 4℃ for a short period of time (use up within 10 days).
Applications	Scope of application: a wide range of plant seeds. DNA released from sample lysis: only used as PCR template. The kit can be used for the following purposes: transgenic seed identification, plant genotyping, etc.
Product Composition	Buffer SP1: Provides the necessary environment for plant seed lysis reaction. Buffer SP2: Neutralize the lysis products so that they do not affect the subsequent PCR reaction. 2× Seed PCR Mix: contains Taq DNA Polymerase, dNTPs, MgCl2, reaction buffer, PCR enhancer, optimizer and stabilizer, etc. During the PCR reaction, just add the appropriate lysis mixture, primer and ddH2O into the 2× Seed PCR Mix for PCR reaction. 6× DNA Loading Buffer: The Loading Buffer does not contain SDS, and it is recommended to use the 6× DNA Loading Buffer that comes with the kit for agarose gel electrophoresis in order to obtain good electrophoresis results. Do not use the SDS-containing Loading Buffer, otherwise there will be a large trailing bright light in the lane during electrophoresis, which will affect the results of the experiment.
Features	In order to meet the needs of different detection experiments, this kit can be used for experiments with various types of samples (e.g., intact seeds, microtissue cuttings, or ground samples of multiple seeds). Among them, for seed samples that still need to undergo germination, one can choose to cut trace tissue cut pieces (1-5 mg) other than the seed embryo for experiments; for experiments that need to sample a large number of samples for detection, one can choose to mix and grind multiple samples into particles with a diameter of 0.5 mm or so and then carry out the experiments (a minimum of 0.1% of the target samples can be detected). The process of releasing genomic DNA from the lysis reaction system can be completed within 5-10min, without the need for other processes to remove proteins, RNA or secondary metabolites, and the released trace DNA can be used as a template for PCR reaction.
Advantages	Eliminates the need for time-consuming and expensive DNA purification. Small sample size, only need to take a single seed for the experiment. No special treatment such as grinding and crushing is required, so it is easy to operate. Optimized PCR system for higher specificity and better tolerance of PCR inhibitors.
Precautions	1. This kit is only suitable for seed samples with low content of polysaccharides and polyphenols. For samples with high content of polysaccharides and polyphenols, please choose the Plant Seed Direct PCR Plus Kit. 2. Pay attention to the cleanliness of the experimental tools and experimental practices to avoid cross contamination between samples. 3. Try to use seeds that are less than 1 year old for the experiment. If the seeds have been stored for more than 1 year or if the seeds are particularly dry, break the seed coat or use cracked seeds when lysing. 4. If precipitate is precipitated from Buffer SP1, leave it at 37℃ until the precipitate disappears and shake the solution well before use. 5. 2× Seed PCR Mix should avoid repeated freezing and thawing, otherwise the PCR efficiency will be affected. 6. If the ambient temperature is too high, the 2× Seed PCR Mix may become turbid, place it on ice for 1-2min, wait until the solution is clarified, and mix it upside down for 3-5 times before use. 7. Do not use SDS-containing Loading Buffer during electrophoresis, otherwise a large trailing bright band will appear in the lane, which will affect the results of the experiment.