PRODUCT
TtCsm6
Cat#
CASPT-021
Source Organism
Thermus thermophilus
Expression Host Organism
E. coli
Tag Type
His
Sequence
full length
Introduction
Recombinant Thermus thermophilus protein TtCsm6 was expressed in E. coli cells using a C-terminal His tag.
Size
50 ug/100 ug/Bulk Customize
Alternate Names
CRISPR system endoribronuclease Csm6, CRISPR type III-A associated protein Csm6
UniProt Number
Q53W17
Overview
TtCsm6 is an endoribonuclease specific for single-stranded RNA that consists of the N-terminal CARF domain and the C-terminal HEPN domain. The ribonuclease active site is formed by the HEPN domain dimer. TtCsm6 belongs to the Class 1 type-III-A CRISPR-Cas system (1). Upon activation of the CRISPR ancillary ribonucleases Csx1/Csm6, the HEPN active site degrades invasive RNA non-specifically. TtCsm6 is activated by the cyclic oligoadenylate second messengers that was converted from ATP by the Cas10 subunit of the Type III interference complex (2).
Accession Number
WP_011229148
Formulation
Protein stored in 50 mM sodium phosphate, pH 7.5, 300 mM NaCl, 150 mM imidazole, 1 mM DTT, 10% glycerol
References
1. Niewoehner, O. et al. 2016. Structural basis for the endoribonuclease activity of the type III-A CRISPR-associated protein Csm6. RNA 22(3):318-329.
2. Niewoehner, O. et al. 2017. Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548.
2. Niewoehner, O. et al. 2017. Type III CRISPR–Cas systems produce cyclic oligoadenylate second messengers. Nature 548(7669): 543-548.
Storage and Stability
Store at –70°C. To avoid repeated handling and multiple freeze/thaw cycles aliquot product into smaller quantities.
Concentration
1.0 μg/μl
18.18 pmol/μg
18.18 pmol/μg
Purity
>90%
Molecular weight
Calculated: 52.7 kDa
Observed: ~50 kDa
Observed: ~50 kDa
Recommendation
Always include RNase inhibitor at recommended concentrations in Cas enzyme reactions.
